WATERS 2487 DUAL ABSORBANCE DETECTOR CALIBRATION

In this article we will learn how to how to calibrate WATERS 2487 DUAL ABSORBANCE DETECTOR.
we will learn two important parameters to calibrate DETECTOR, which is wavelength accuracy &  linearity response.

 A) Wavelength Accuracy:

Standard Preparation:

Take 100 mg of  caffeineà100 ml. à 1ml à 100ml with Methanol.

Parameters:

Mobile Phase:  Methanol HPLC grade       Column:  MERCK C18 (250 mm x 4.6 mm. 5 micron)

Wavelength:  252- 264 nm with 2 nm increment.      Flow Rate:  1 ml / min.   

Injection Volume: 20 ml.

Sr.No.	Wavelength (nm)	Area	Mean
1	250	121413	127599.40
2	252	130048
3	254	132026
4	256	130516
5	258	123994

Largest peak response is observed at  254 nm. (Limit: 254 nm  ± 2 nm)

B) Check for Linearity of response  :

Sr. No.	Injection volume	Area
1	10 ug/ml	132358.0
2	60 ug/ml	255619.0
3	140 ug/ml	384955.0
4	220ug/ml	509304.0
5	330ug/ml	636119.0

Remarks: Satisfactory / Not satisfactory.

if you have more questions about detector calibration you may visit.

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